SEQanswers - View Single Post - Agilent SureSelect XT and Illumina flowcell saturation

anjama · 02-20-2019, 06:45 AM

Does anyone know if the SureSelect XT kits have enough probes in a single reaction to saturate a single lane in an Illumina HiSeq flowcell (200-400 million reads) with unique fragments? My concern is that if it doesn't, then my sequencing will consist of excessive pcr duplicates. Thanks.

02-20-2019, 06:45 AM	#1
anjama Member Location: Florida Join Date: Mar 2016 Posts: 15	Agilent SureSelect XT and Illumina flowcell saturation Does anyone know if the SureSelect XT kits have enough probes in a single reaction to saturate a single lane in an Illumina HiSeq flowcell (200-400 million reads) with unique fragments? My concern is that if it doesn't, then my sequencing will consist of excessive pcr duplicates. Thanks.