View Single Post
Old 09-14-2020, 02:56 AM   #1
al_igator
Junior Member
 
Location: UK

Join Date: Sep 2020
Posts: 3
Default RNA-seq library prep from ~100cells

Hi all,

I'm entirely new to RNA sequencing and am looking for some advice re: library prep with low cells numbers.

I've started a new project and am looking to do bulk RNA seq (Illumina) on different populations of FACS sorted hematopoietic stem and progenitor cells from mouse bone marrow. Some of these populations are estimated to be 50-200 cells per sample.

I'm aiming to do the RNA extraction and library preparation myself but there seems to be loads of different kits available for low (or ultra low) RNA input. Would be great to know what people have had success with before!

Any advice, experience or recommendations for library prep with ~100 HSPCs would be massively appreciated.

Thanks!
al_igator is offline   Reply With Quote