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Old 01-10-2011, 05:15 AM   #2
Location: Aarhus, Denmark

Join Date: Mar 2008
Posts: 57

Why not simply use the "Index PE Adapter Oligo Mix" from the Illumia 12-plex kit instead of the normal "PE Adapter Oligo Mix" supplied with the mate-pair kit? Then you can build in the indexes (index #6 and #12 for 2-plex) during the PCR.
I have produced mate-pair libraries with insert sizes up to 10 Kb - but not indexed. But I would like to know if it works :-)
Cheers, Jakob
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