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Old 11-04-2012, 05:10 PM   #1
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Location: CA, USA

Join Date: Nov 2012
Posts: 1
Default Disagreement between bioanalyzer and agarose gel for PCR amplicon library


We are setting up for an illumina MiSeq run using a PCR amplicon-based library (adding the Illumina adaptors using nested PCR). Our results look quite clean via agarose gel (expected size ~310nt), but when our facility ran bioanalyzer we had quite variable results (ranging between 400 and 500nt, depending on the sample). There are seven samples, each with a different barcode, labeled 1-7.

I'm attaching both the gel and the bioanalyzer report - has anyone seen anything like this before?

Attached Images
File Type: jpg gel result.jpg (63.9 KB, 129 views)
Attached Files
File Type: pdf Bioanalyzer result.pdf (431.4 KB, 173 views)
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