Hi All,
Just wondering what your experiences are in regards to over vs under drying SPRI beads during NGS.
I am currently using Nextera XT with Ampure XP beads. I give a 5-10min RT dry but I still get very low yield 1-4ng/ul in my libraries.
Do you feel if I extended this to 15mins as per protocol I may get better yield? How do I know if I have over dried the beads?
Just wondering what your experiences are in regards to over vs under drying SPRI beads during NGS.
I am currently using Nextera XT with Ampure XP beads. I give a 5-10min RT dry but I still get very low yield 1-4ng/ul in my libraries.
Do you feel if I extended this to 15mins as per protocol I may get better yield? How do I know if I have over dried the beads?
Comment