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Old 06-08-2011, 08:34 AM   #3
Heisman
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Location: St. Louis

Join Date: Dec 2010
Posts: 535
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Quote:
Originally Posted by kwaraska View Post
There is a kit from Kapa for library quantification that works well.

We are using a homemade kit using Kapa SYBR reagents, primers, and PhiX as a standard curve. We tried the Kapa kit and it was just as good as ours, but ours is less expensive.
Thanks for the reply! How do you design your primers for the paired-end preps? I'm a rookie at qPCR so please excuse my ignorance.
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