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Old 06-08-2011, 10:14 AM   #1
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Location: USA

Join Date: Apr 2010
Posts: 102
Default help with TruSeq

I have recently used TruSeq kit for making 8 of Brassica RNAseq libraries and after amplification (15 cycles) and running on the gel (1% Agarose) i found that 5 out of 8 libraries have a broader range of fragments (smear) compared to the rest of the 3 libraries. I don't know why and the only reason i can think of possible over-amplification. But if it is so why only 5 and not all 8 have this problem. Any help is appreciated.

I have attached the picture of the gel for easy understanding.

Attached Files
File Type: pdf TruSeq libraries 1-8.pdf (592.8 KB, 49 views)
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