Hi everyone
we're hoping to start an RNASeq run on the Illumina HiSeq SQ in the next few weeks. I recently came across the ERCC Spike-In Control, which lets you add known amounts of mRNA to your sample.. I'm interested in using the ExFold mixes, which are two mixes with different amounts of the same mRNAs.
It looks like it could be really useful! I've had a look at the "Synthetic spike-in standards for RNA-seq experiments" paper, but I have little background in bioinformatics... what they've done seems too complicated for me to repeat.. Does any one know a program which can take advantage of spike-in controls (eg. for normalisation, checking quality of the run)?
Any advice will be greatly appreciated
Thanks in advance,
Suthira
we're hoping to start an RNASeq run on the Illumina HiSeq SQ in the next few weeks. I recently came across the ERCC Spike-In Control, which lets you add known amounts of mRNA to your sample.. I'm interested in using the ExFold mixes, which are two mixes with different amounts of the same mRNAs.
It looks like it could be really useful! I've had a look at the "Synthetic spike-in standards for RNA-seq experiments" paper, but I have little background in bioinformatics... what they've done seems too complicated for me to repeat.. Does any one know a program which can take advantage of spike-in controls (eg. for normalisation, checking quality of the run)?
Any advice will be greatly appreciated
Thanks in advance,
Suthira
Comment