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Old 05-08-2013, 09:50 AM   #1
Anna IT
Junior Member
 
Location: Italy

Join Date: May 2013
Posts: 1
Smile DNA extraction from Saliva

Hi everybody!!
I have to extract DNA from Saliva, in order to realize some ION Torrent PGM libraries. I'd like to ask if you know which is the best kit for DNA extraction. In the past we used the Oragene extraction protocol, but our samples were full of proteins. So we have thought about using the DNA mini blood kit, that is compatible also with saliva. In fact our aim is to have a good concentration, but also a great quality of DNA samples. One of the limits of this kit is the cost, because it's really expensive. So I tried to find out something similar, but less expensive. I've read something about the NORGEN DNA saliva isolation kit. Have you ever used it? Do you know other kits that I can use?
Thank you in advance for answering!
Anna
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