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Old 07-31-2017, 07:23 AM   #2
HESmith
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Location: Bethesda MD

Join Date: Oct 2009
Posts: 503
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Adapter ligation is a concentration-dependent reaction. If enrichment is performed first, then the amount of one of the reactants (fragments) becomes low, which reduces the efficiency of the reaction (i.e., less library).

P.S. - It's not necessary to post the same question in multiple topic areas.
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