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Old 01-17-2018, 05:01 PM   #1
Location: Hong Kong

Join Date: Jul 2017
Posts: 14
Question Reducing use of NEB reagents in Nanopore barcoding (96) genomic DNA kit

Dear All,

Recently I used the nanopore 1D PCR barcoding (96) genomic DNA kit to construct the multiplex library. And I found that I need to use the standard volume of NEB reagent for the End prep and Ligation of Barcode Adapter of each of the 96 samples. I wonder if it is really necessary to prepare each of the 96 samples in a way that likes preparing the single library? I think I can somewhat reduce the reaction volume of each sample as they 96 samples would be pooled together finally.

So I want to know if someone have similar experience on this?

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