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Old 01-18-2018, 12:13 AM   #5
liaoyunshi
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Location: Hong Kong

Join Date: Jul 2017
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Quote:
Originally Posted by Edinburgher View Post
Molecularly, it should work. However, validation studies need to be in place before you decide to cut the reaction size. RNA access kit is about $14K for 96 reactions. If one can manage to survive with half of the size, imagine how much he/she can save? I am also surprised by the fact that only a tiny bit (0.1-0.2 ul out of 30 ul) is needed for the actual sequencing.
I totally agree with you, I cannot imagine why we should prepare 96 samples respectively in a way for a single sample library, and finally you need only 1/96 of each sample to be pooled.
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