Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Bowtie2 exact match and same length

    Hi,

    I have 8 fastq files with small RNA sequencing data and I would like to allign the sequences to a reference (.fa), thereby removing the sequences that do not allign perfectly to the reference sequences. However, the condition is that the perfect alligned seqeunces have the same length as the reference sequences to which they were alligned to. Example (not the real data, but to show what I mean):

    Ref sequence (.fa):
    CAATCGATCGATGCTAGTC

    sample sequences (.fastq):
    GCAATCGATCGATGC
    CAATCGATCGATGCTAGTC
    AATCGATCGATGCTAGTC
    GTACCATCGACT

    Expected output from bowtie (.sam):
    CAATCGATCGATGCTAGTC

    This is the command I used:
    bowtie2 -L 6 -i S,0,0.5 --rdg 1,6 --rfg 1,6 --norc --score-min C,0,-1 -p 8 -x "INDEX" (input_file.fastq) > (output_file.sam)

    The command runs and produces output, however not exactly what I expected.

    Bowtie2 output from command:
    CAATCGATCGATGCTAGTC
    AATCGATCGATGCTAGTC

    How can I change the bowtie2 command to remove perfect alligned sequences that are not the same length as the reference sequences? Or use Samtools to remove the smaller/longer sequences?

    Thanks in advance!
    Last edited by Aquilifer; 04-09-2018, 06:19 AM.

Latest Articles

Collapse

  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin


    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
    Yesterday, 07:01 AM
  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
55 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
52 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
45 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
55 views
0 likes
Last Post seqadmin  
Working...
X