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Old 06-09-2014, 07:19 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
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They do not look like bubbles that you are reffering. They could be remaining non-target digested DNA themselves or their amplification products or both. If size-selection was done on Pippin instrument, my previous statement would not be true. Maybe the bioanalyser reagents and run (specially ladder) should be inspected more closely for further explanation in first step. The answer might be in the modified protocol that have been used to prep libraries.

Last edited by nucacidhunter; 06-09-2014 at 07:24 PM.
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