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Old 05-17-2020, 10:39 AM   #4
Senior Member
Location: East Coast USA

Join Date: Feb 2008
Posts: 7,079

I suggest you try "" from BBMap suite instead. A guide is available. You can trim out all these things in one pass.

I am not a trim galore user but you may be able to additional specify poly-A and poly-G there as well.
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