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Old 09-09-2015, 09:54 AM   #3
avilella
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As an additional note to the previous answer, the duplicate rate will increase the deeper you sequence a given sample.

The curve of total reads and total distinct reads will depend on the diversity, as depicted here:

http://smithlabresearch.org/software/preseq/challenge/

There are ways to improve the duplicate rate of WGBS by using more recent commercial conversion kits from other vendors, generally categorized as post-BS kits.

Last edited by avilella; 09-13-2015 at 02:19 AM.
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