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Old 02-24-2016, 04:12 AM   #3
Alex852013
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Location: Germany

Join Date: Jan 2013
Posts: 17
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Could you please write what part you mean? If you start with the first few words of the text it will be easier to find. Thanks.

I used the ERCCs, because i think that my factor might upregulate a lot of genes. If it does so, the normalization without ERCCs will destroy the result.
This means upregulated genes might be interpreted as downregulated.

Figure 1 (without ERCCs) and figure 2A (with ERCCs) in paper http://www.sciencedirect.com/science...92867412012263 shows the problem clearly. I'm afraid there is no other way to exclude this, but ERCCs.
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