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Old 02-24-2016, 04:22 AM   #4
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Location: Geneva

Join Date: Feb 2012
Posts: 175

Of course, in some cases (as wrote blancha in the other discussion), it may help... Personally, I never used and I wanted to have to opinion of the SEQanswers community. It seems, depending on the project, they can bring more issues than they can solve (but again, depending on the project).

I was referring to the the part where the authors say that their normalization is robust when applied to a set of control genes, or set of replicates, while it gave reasonable results using the ERCC spike-ins...
SylvainL is offline   Reply With Quote