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Old 02-25-2016, 12:40 AM   #11
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Location: Geneva

Join Date: Feb 2012
Posts: 175


I totally understand your points and why you want to use the ERCC spike-ins. Probably in your case, it is really necessary. But I believe it is important to look how people did their analysis to be sure their normalization method really brings a plus... Unfortunately, I do not have time right now, but I will re-analyze the data of this paper, using different pipelines to get my own idea about this spike-ins normalization...
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