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Old 06-07-2010, 07:26 AM   #51
Location: Montreal

Join Date: Oct 2009
Posts: 63

The illumina RNA protocol uses random hexamers to amplify the RNA. The thing is they are not 100% random so the beginning looks skewed for base composition, but that's because of the amplification.

For mapping it's no problem. For assembly it might confuse some assemblers. (When assembling I would trim the 5' of RNA, not for mapping)
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