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Old 03-29-2017, 11:06 AM   #11
eingabe_ausgabe
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Location: sacramento, ca

Join Date: Mar 2017
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Quote:
Originally Posted by pmiguel View Post
So, correct me if I'm inferring the wrong thing, but the figure from the paper you link to is saying that incomplete lysis results in some of the RNA fractionating with debris that is removed from the RNA prep?
http://www.nature.com/nprot/journal/...16.090_F2.html

Does "insufficient lysis" mean cells that are only partially lysed such that only the small RNAs are released and the rest is washed away with the cells? My original interpretation was that, perhaps, some of the cells were recalcitrant due to the nature of the tissue and, thus, took longer to break apart and were subject to endogenous RNases for longer periods of time?

Also, yes, well aware of the insect 18S/28S single peak.

Thanks.
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