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Old 03-29-2017, 11:31 AM   #12
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
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Quote:
Originally Posted by eingabe_ausgabe View Post
http://www.nature.com/nprot/journal/...16.090_F2.html

Does "insufficient lysis" mean cells that are only partially lysed such that only the small RNAs are released and the rest is washed away with the cells? My original interpretation was that, perhaps, some of the cells were recalcitrant due to the nature of the tissue and, thus, took longer to break apart and were subject to endogenous RNases for longer periods of time?

Also, yes, well aware of the insect 18S/28S single peak.

Thanks.
Yes, your interpretation is more likely.

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Phillip
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