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Old 03-21-2019, 08:05 AM   #9
ShyN
Junior Member
 
Location: Mountain View

Join Date: Feb 2019
Posts: 7
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Quote:
Originally Posted by Ahutch08 View Post
You need to load the run into BaseSpace to see SAV metrics for iSeq. Ability to view SAV metrics on instrument is coming in later software update.

Did you determine whether your input library concentration was too low or too high?

We're going to try to so BaseSpace now - we used a local application before but I think its time we give base space a chance.

We ended up concluding that our loading concentration was wayyy too low. So out PF was low because of this. Our %Q30 was high because out of the samples that passed (low as they were), most of them were useable.

Thanks everyone for your input!

Last edited by ShyN; 03-21-2019 at 08:10 AM. Reason: Incorrect response
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