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Old 04-01-2019, 06:39 AM   #12
a.boese
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Location: Canada

Join Date: Mar 2019
Posts: 1
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I recently started using the iSeq and I had the same problem. I felt that my library prep concentration was too high and I got these results by changing the concentrations (I was using PCR products fragmented with the NEBNext Ultra II FS library prep kit):

200pM % PF 39.26% AVG %Q30 82.64%
100pM % PF 67.54%; AVG %Q30 89.15%
50pM %PF 73.92%; AVG %Q30 91.81%
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