View Single Post
Old 09-02-2010, 05:58 AM   #20
Junior Member
Location: USA

Join Date: Aug 2010
Posts: 4

we've been having problems with SV lots (93768220 and 93776220). they look like the emPCR is the issue though no obvious broken reactors. the attached 1/8 Ti bacterial genomic was deemed "good" by the Roche engineer we talked to (though i disagree). we've sequenced the same type of sample/prep from this PI with lot 9378740 and beautiful distribution with avg of 500 bp. now, hardly any reads above 400 bp with a very odd peak at 120 bp. we've submitted RunReports etc to support with only the normal "we've received your message" response. any others have this lot (especially *8220)?
Attached Images
File Type: jpg good_region.jpg (86.5 KB, 40 views)
gallus is offline   Reply With Quote