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Old 04-19-2012, 06:20 PM   #1
guz
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Location: Boston, MA

Join Date: Oct 2009
Posts: 7
Default Strange peak in total RNA extractions from brain tissue

We have extracted total RNA from frozen brain tissue. To extract RNA, we homogenized 30-50mg tissue with Trizol then added chloroform for phase separation and lastly did a buffer exchange using the miRNeasy kit. We did not perform DNase treatment. We are seeing a large peak at around 100-150nt from the bioanalyzer for all 40 brain samples we used regardless of RNA integrity numbers (see attachment). In fact most RINs are high. Has anyone else seen this? Is this degraded RNA? If it was degraded RNA, wouldn't it be a smear? Or is it possible it is DNA?
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File Type: png Total RNA from Brain.png (58.2 KB, 109 views)
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