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Old 04-20-2012, 04:05 AM   #2
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Location: Purdue University, West Lafayette, Indiana

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Those are your small RNAs. tRNA, 5S, 5.8S, etc. Many RNA prep kits do not efficiently isolate them, but Trizol can -- depending on your post-extraction concentration/purificiation method. "miRNeasy" would stand for "micro RNA easy". So one would expect lots of small RNA there.

In some samples isolated with Trizol we see small RNA peaks so high that the BioAnalyzer software balks and refuses to give a RIN until you suppress certain errors it generates.

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