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Old 06-04-2012, 07:14 AM   #6
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Location: Germany

Join Date: Jun 2012
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Default Bioanalyzer band shift 150 -> 100 nt

Dear all, we encountered shift at the similar band size. We isolated total RNA from neuronal cell culture using combination of QIAzol Lysis reagent (QIAGEN) and Direct-zol RNA MiniPrep (Zymo Research). Quality of the isolated RNA was checked on Expirion (Bio-Rad Laboratories) (direct competitor of Agilent Bioanalyzer). Under control conditions, we have observed relatively defined double-band at ~ 150 nt (green). Upon an experimental treatment the double-band weakened and new broad band with most intensive part at ~ 100nt appeared (orange, see image attached). It was consistent in all cases when treatment was used. Do you have an idea what class of RNAs could be responsible for the shift and why could this shift occur? Thank you for response!
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File Type: png Shift in smallRNAs 02.png (48.2 KB, 48 views)
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