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Old 11-07-2010, 06:45 AM   #2
KevinLam
Senior Member
 
Location: SEA

Join Date: Nov 2009
Posts: 198
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How are u assigning the reads?
I had the same prob in 3 plus. Due to multiple reasons.

I used wrong library in setup. ( check if majority of reads are in a particular barcode.

Did u allow 1 mismatch?

Check w ur FAS if there's a better hamming sphere file.
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