Hi, All
I just got a problem when splitting 4M reads from read1 in one lane (Illumina GA) into 1-million chunk. I tried:
maq fastq2bfq -n 1000000 s_1_1_sequence.fastq s_1_1_sequence
and I got 5 output files:
[email protected]
[email protected]
[email protected]
[email protected]
[email protected]
I understand these four files:
[email protected]
[email protected]
[email protected]
[email protected]
are the output split files. But I don't know what "[email protected]" is.
I tried this command on read 2 from the same land and got the same problem. It output "[email protected]". It also happens to all the other flow cells.
Also, when I changed it to 2-million chunk, I still got this @1.bfq file.
I checked the size of this @1.bfq file and it is similar to the other @1000001. bfq or other .bfq output files.
Can anybody tell me what this @1.bfq file is? Should I remove it for further mapping?
Thanks in advance
-Cliff
I just got a problem when splitting 4M reads from read1 in one lane (Illumina GA) into 1-million chunk. I tried:
maq fastq2bfq -n 1000000 s_1_1_sequence.fastq s_1_1_sequence
and I got 5 output files:
[email protected]
[email protected]
[email protected]
[email protected]
[email protected]
I understand these four files:
[email protected]
[email protected]
[email protected]
[email protected]
are the output split files. But I don't know what "[email protected]" is.
I tried this command on read 2 from the same land and got the same problem. It output "[email protected]". It also happens to all the other flow cells.
Also, when I changed it to 2-million chunk, I still got this @1.bfq file.
I checked the size of this @1.bfq file and it is similar to the other @1000001. bfq or other .bfq output files.
Can anybody tell me what this @1.bfq file is? Should I remove it for further mapping?
Thanks in advance
-Cliff
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