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Old 06-18-2015, 04:24 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232
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I think they have Taq polymerase in End repair mix. During incubation at 65C Taq actively adds A to 3' ends, while the other mesophilic enzymes used for end repair and 5' phosphorylation are denatured and deactivated.
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