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Old 06-18-2015, 09:29 PM   #3
Location: Los Angeles, CA

Join Date: Dec 2014
Posts: 30

That is what I am thinking, too, but I have never seen a library prep protocol using it. Everyone seems to be using Klenow 3 - 5 exo for A-tailing and I have seen various combinations of enzymes for end repair.

Could Klenow be working simultaneously with the other enzymes and then they're all denatured?

Last edited by Ingeneious; 06-18-2015 at 09:32 PM.
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