View Single Post
Old 12-09-2015, 07:20 AM   #6
mama
Junior Member
 
Location: paris

Join Date: Sep 2008
Posts: 8
Default

We are currently using the Nextflex small RNA Seq kit v2 and we get huge amount of adapters dimers in our libraries. We use a PAGE TBE 10% gel after the PCR and most of the time the band at 150 bp is not as sharp and tight as Bioo scientists says. We retrieve this band at 150 bp and up to 80% of this band is adapters dimers (130-140bp). It means that adapters dimers could also migrate at 150 bp.
mama is offline   Reply With Quote