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Old 08-24-2016, 02:41 PM   #5
yuehuang
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Location: us

Join Date: Jan 2013
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Quote:
Originally Posted by adam.geber View Post
yuehuang, would you mind sharing your findings here when appropriate? I use a different method to make 16S libraries but have experienced similar sizing discrepancies in the past so I'm interested to know how you and Bioo interpret these results.
Adam, I used to using V1-V3, couldn't get the exactly pure one band peak either, much more impure than the one I showed here. But I used Pippin Prep to pick the correct size libraries, which help us get decent MiSeq data. It's the first time I used V4, I'm following Brad's suggestion to see what's gonna happen. But I'm working on human microbiome, other than gut microbiome, I can't be sure if my samples contain bacteria. Anyway, I will update this thread if those libraries get sequenced.
Good luck!
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