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Old 04-11-2017, 06:39 AM   #1
Location: Warsaw

Join Date: Mar 2014
Posts: 17
Default Read lenght in 16S RNA data


My amplicon after assembly shoud have approx. 460bp. Do I have to analyze only 460bp products or can I also analyze some shorter/longer amplicons. If yes, how to set a specific lenght range/limit. For example I have 200000 reads per sample, almost 52% of them are shorter than 460bp, but only 11% are shorter than 440bp. I think that those reads also have an importance and value, but how to set the range?

Please help me.
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