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Old 07-05-2017, 12:21 PM   #1
Junior Member
Location: USA

Join Date: Oct 2016
Posts: 3
Unhappy Short mixed amplicon libraries

I'm interesting in sequencing libraries composed of multiplexed PCR products with amplicon lengths running from 50 - 150 bp. A colleague expressed concern about sequencing bias for shorter amplicons on the Illumina platforms (likely will want to use MiSeq).

Does anyone have experience sequencing libraries of short, mixed-length amplicons/insert sizes? If so, can you comment on the level of sequencing bias? If sequencing bias was a problem, do have a good working solution (e.g., pooling amplicons of different lengths at different relative molarity)?
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