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Old 07-11-2017, 05:47 AM   #7
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 243

My primary type is 16s v4 (~300bp). I've mixed up to 30% of a run with ITS2 (~250-400bp) or custom ~150bp amplicons with no issues and no noticeable decrease in reads for the longer amplicons on the run but I'm looking for a minimum per sample rather than average
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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