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Old 10-24-2017, 06:48 PM   #1
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Location: Canada

Join Date: Sep 2017
Posts: 3
Default "Stranded" data may actually be unstranded?


I have some paired-end RNA-seq data that I was told were "stranded", but nothing about whether it's RF or FR.

I aligned some randomly sampled pairs of reads to the reference genome of the organism. I see that 50% of the read 1s map to the "+" strand, the other 50% to the "-" strand. Same as the read 2s.

Does this mean that the data is actually unstranded?

P.S. I can't get QoRTs to work on my data, so I can't use it to tell me whether the data is stranded or not.

Thank you!
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