View Single Post
Old 10-24-2017, 11:41 PM   #2
Michael.Ante
Senior Member
 
Location: Vienna

Join Date: Oct 2011
Posts: 123
Default

Hi DMNO,

did you take the transcripts' strand-orientation into account? Try using RSeQC's infer-experiment tool. It gives you a good way to assess if your library was stranded and in which orientation.

Cheers,

Michael
Michael.Ante is offline   Reply With Quote