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Old 05-24-2018, 11:19 AM   #9
donnyKerabatsos
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Location: Boston, MA

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This paper describes how to combine these steps in one reaction using individually-ordered (i.e. cheap) enzymes and buffers: T4 PNK, T4 polymerase and taq, same as @ECO suggests.

Library Preparation and Multiplex Capture for Massive Parallel Sequencing Applications Made Efficient and Easy
Mårten Neiman, Simon Sundling, Henrik Grönberg, Per Hall, Kamila Czene, Johan Lindberg , Daniel Klevebring
PLoS One
http://journals.plos.org/plosone/art...l.pone.0048616

Last edited by donnyKerabatsos; 05-24-2018 at 11:21 AM.
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