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Old 12-21-2018, 03:09 PM   #13
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232
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Twist Bioscience Exome protocol does not claim end-repair at 65C. It must have been a miscommunication. Most enzymatic library prep kits include fragmentation and end repair at 20-35C and A-tailing at 65C.
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