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Old 10-03-2019, 07:20 AM   #4
Junior Member
Location: Madison

Join Date: Feb 2019
Posts: 8


I only use Qubit for final library quants after PCR and AMPure cleanup; I've not done DNA quants after RNA extraction (our samples are either samples that are acquired from retail sources of from collaborators who use standard RNA purification, with DNAse I/Turbo DNAse treatment. Sample integrity is assayed bioinformatically after sequencing and use of an RNA-Seq aligner program (Bowtie, etc.)

Is the main issue with DNAse removal something to do with the sample types or the data that you are trying to acquire?
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