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Old 10-08-2019, 12:12 PM   #9
kumard
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Location: Midwest USA

Join Date: Jun 2019
Posts: 6
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Quote:
Originally Posted by olafblue1955 View Post
Hi,

I only use Qubit for final library quants after PCR and AMPure cleanup; I've not done DNA quants after RNA extraction (our samples are either samples that are acquired from retail sources of from collaborators who use standard RNA purification, with DNAse I/Turbo DNAse treatment. Sample integrity is assayed bioinformatically after sequencing and use of an RNA-Seq aligner program (Bowtie, etc.)

Is the main issue with DNAse removal something to do with the sample types or the data that you are trying to acquire?
I am a newbie in RNASeq. Most of the RNA-library prep kits talk about using DNA-free RNA. That is why I am concerned about the residual DNA.
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