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Old 10-30-2019, 07:20 AM   #1
cement_head
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Location: Oxford, Ohio

Join Date: Mar 2012
Posts: 250
Default (2x) Final Bead Clean-Up

Hello,

I have a general question regarding NGS library prep protocols; many of the protocols suggest using TWO rounds of bead clean-up at the end of the protocol. I am curious as to why TWO (2) rounds of clean-up. Shouldn't one "proper" iteration (round) be enough? Or are the companies worried about primer dimers so much that they are trying to CTAs and suggest two rounds?

Thanks-in-advance...

Last edited by cement_head; 10-30-2019 at 07:21 AM. Reason: clarification
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