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Old 10-30-2019, 02:22 PM   #2
jdk787
josh kinman
 
Location: Austin

Join Date: Apr 2014
Posts: 69
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I have seen many PCR Free protocols recommend two cleanups to be sure to get rid of any unincorporated adapters which are hard to detect and known to cause index hopping.

This could also be needed after PCR for protocols that use PCR primers that include index sequences.
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Josh Kinman
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