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Old 01-26-2020, 09:19 PM   #1
BDGLifescience
Junior Member
 
Location: Delhi

Join Date: Jan 2020
Posts: 2
Arrow RNASeq, Whole Genome & MetaGenomics Training in Dubai

2-day Technical Training in NGS Data Analysis | DNA, RNA & MetaGenomics in Dubai
When: 6-7 April 2020
Where: Executive Lounge, Business Centre, Level 30, The H Hotel-Office Tower, #1 Sheikh Zayed Road, Dubai, United Arab Emirates
This will be our 6th event in Dubai

Event Webpage

PRACTICAL APPLICATION: In the workshop, we will perform 3 experiments to analyze the example RNASeq, DNASeq & MetaGenomics data. Hands-on exercises will be performed individually using software tools on your own laptops. No prior software experience is required.
SCHEDULE | DAY 1
EXPERIMENT 1

In this lesson, we will focus on the SNPs and Variant Calling (VC) of the DNASeq data and cover:
  • Deal with the raw data for quality evaluation and filtering
  • Map reads to the reference genomes, call SNPs and predict their phenotype consequence
  • Alignment to a reference genome, SNPs and In-dels Calling of next-generation sequencing DNA data

SCHEDULE | DAY 2
EXPERIMENT 2

In this lesson, we will focus on the Differential expression of Genes (DEGs) of the RNASeq data and will cover:
  • Upload Data
  • Convert File Format
  • Align Reads To Reference Genome
  • View The Mapped Reads In JBrowse
  • Count Reads Per Gene
  • Test For Differential Expression
  • Differential Gene Expression
  • MDS Plot
  • MA Plot
  • Volcano Plot
  • Parallel Coordinates and Heatmap
  • Investigate Differentially-Expressed Genes
  • Chord Diagram

EXPERIMENT 3
In this lesson, we will focus on the MetaGenomics Analysis in 16S rRNA | Amplicon Sequencing Data Analysis (OTUs) and will cover:
  • File Optimization- Loading of data & optimizing it according to our experiment.
  • Quality Control- Check sequencing quality
  • Sequence Alignment- Grouping/Alignment of reads
  • Extraction of Taxonomic Information- Operational Taxonomic Units (OTUs)- Clustering Sequences
  • Visualization- Examine diversity of species/taxa present within a single sample, Comparison of species/taxa diversity between two samples & Taxonomic Diversity- How many species are in a sample? What are the differences in species between samples?
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