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Old 01-31-2020, 01:31 PM   #3
aesterle
Junior Member
 
Location: Fort Collins, CO

Join Date: Jan 2020
Posts: 2
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Hey there, microgirl:

I was wondering if you tried what luc suggested and if this worked.

Also, do you have a low annealing temperature on your PCR cycles? I figure it's stayed the same, but since other things have been changed, maybe the thermocycler programming needs to be altered as well.

I've only been doing library prep for around 1.5 yr, but it surprised me how low the annealing temps in a lot of protocols are set to. We anneal at 50C!
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