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Old 03-05-2020, 01:09 PM   #1
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Location: US

Join Date: Mar 2020
Posts: 1
Question Weird trace in Next-Seq run


I have an issue with a recent Next-Seq run and I'm wondering if someone might have any insight. In this run, we pooled together two libraries that were prepared as a customization of the 10x 3' protocol.
The first 25 base pairs seem to be okay, but then there is a stretch of about 75bp of T's. This is not something we were expecting in our library.
I was thinking maybe the adaptor ligation for one of these libraries didn't work, and that possibly messes up the sequencing run? Any other idea would be greatly appreciated since at this point we're not sure what went wrong.
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