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Old 03-05-2020, 04:02 PM   #3
josh kinman
Location: Austin

Join Date: Apr 2014
Posts: 69

Originally Posted by luc View Post
This is expected. You are sequencing through the poly-A tail. You can ignore this part of the data, and sequence only ~30 (?) cycles for the forward read next time.
Correct, I think 10X says to do 28 cycles for R1, then 8 for i7, then 90 or however many cycles you have left for the insert read in R2
Josh Kinman
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