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Old 01-01-2012, 08:17 AM   #1
ETHANol
Senior Member
 
Location: Western Australia

Join Date: Feb 2010
Posts: 308
Default ChIP - shearing with the Covaris S2

I've been doing some optimization work with ChIP and the Covaris S2. I figure some people would probably be interested. All in all it appears to be a great instrument. Fragmentation of the chromatin is really even without bias towards open chromatin which is important for ChIP-seq. My only complaint is that it only takes one sample at a time, so if you have a lot of samples to process it can take a good amount of time. But the reproducibility is worth the extra time.

Anyway, it's too much to post here but it's on my blog at:
http://ethanomics.wordpress.com/2011/11/30/177/
http://ethanomics.wordpress.com/2011/12/28/202/
http://ethanomics.wordpress.com/chip...aris-protocol/

Thanks to Hamid at Covaris for his help. Phenomenal customer support.
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Ethan
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